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Jonathan Kitt
svsniper
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ab69df9c
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ab69df9c
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3 years ago
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Jonathan Kitt
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@@ -33,9 +33,10 @@ Three files are obtained using the Axiom genotyping pipeline:
To read these files, use the following commands:
`axiom_calls <- readr::read_tsv(path_to_axiom_calls_file)`
`axiom_calls <- readr::read_tsv(path_to_axiom_calls_file)`
`axiom_confidences <- svsniper::read_confidences(path_to_axiom_confidences_file)`
`axiom_summary <- svsniper::read_summary(path_to_axiom_summary_file)`
`axiom_summary <- svsniper::read_summary(path_to_axiom_summary_file)`
**Optional step: filter SNPs**
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@@ -43,6 +44,14 @@ You may want to remove SNPs with bad confidence scores, and/or, depending on
the type of analysis you want to run, SNPs with high minor allele frequencies.
In order to filter out SNPs, three functions are available:
`svsniper::count_confidences(axiom_confidences, threshold = 0.15`
This function will count the number of samples with a confidence score above the
defined threshold (defaults to 0.15, the value used in the Affymetrix Axiom
tools), and returns a table as shown below:
`svsniper::count_alleles(axiom_calls)`
This function will return a table as shown below:
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